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triple spots colocalization imaris plugin  (Oxford Instruments)
99
Oxford Instruments triple spots colocalization imaris plugin
Whole-Brain-Scale Mapping of PSD95 and SAP102 (A) PSD95 (green) and SAP102 (magenta) expression in stitched down-sampled images of five coronal sections (S1–S5). Bregma (β) level is indicated. (B) PSD95 and SAP102 synaptome parameters in ARA anatomical subregions. Parameter units are as follows: density, number of puncta per 100 μm 2 ; size, μm 2 ; intensity, mean gray value per punctum (AU × 10 4 ); <t>colocalization,</t> %. (C) Synaptome maps of delineated regions in five sections. Median punctum density (i), intensity (ii), size (iii), and colocalization (iv) for PSD95 (top) and SAP102 (bottom) are shown. Parameter units: density, number of puncta per 100 μm 2 ; intensity, mean gray value per punctum (AU); size, μm 2 ; colocalization, %. (D) PSD95 punctum intensity in delineated subregions of the hippocampus and cortex. CA1, cornu ammonis; CX, isocortex; DG, dentate gyrus. (E) SAP102 punctum intensity in delineated subregions of hippocampus and cortex. (F) Similarity matrix between pairs of subregions (rows and columns). White lines outline three major blocks (cerebrum, brainstem, and cerebellum). Pink box highlights hippocampal subregions. (G) Dendrogram showing hierarchical organization of subregions based on their similarity. Branch tips represent delineated subregions, colored as in (B).
Triple Spots Colocalization Imaris Plugin, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Whole-Brain-Scale Mapping of PSD95 and SAP102 (A) PSD95 (green) and SAP102 (magenta) expression in stitched down-sampled images of five coronal sections (S1–S5). Bregma (β) level is indicated. (B) PSD95 and SAP102 synaptome parameters in ARA anatomical subregions. Parameter units are as follows: density, number of puncta per 100 μm 2 ; size, μm 2 ; intensity, mean gray value per punctum (AU × 10 4 ); colocalization, %. (C) Synaptome maps of delineated regions in five sections. Median punctum density (i), intensity (ii), size (iii), and colocalization (iv) for PSD95 (top) and SAP102 (bottom) are shown. Parameter units: density, number of puncta per 100 μm 2 ; intensity, mean gray value per punctum (AU); size, μm 2 ; colocalization, %. (D) PSD95 punctum intensity in delineated subregions of the hippocampus and cortex. CA1, cornu ammonis; CX, isocortex; DG, dentate gyrus. (E) SAP102 punctum intensity in delineated subregions of hippocampus and cortex. (F) Similarity matrix between pairs of subregions (rows and columns). White lines outline three major blocks (cerebrum, brainstem, and cerebellum). Pink box highlights hippocampal subregions. (G) Dendrogram showing hierarchical organization of subregions based on their similarity. Branch tips represent delineated subregions, colored as in (B).

Journal: Neuron

Article Title: Architecture of the Mouse Brain Synaptome

doi: 10.1016/j.neuron.2018.07.007

Figure Lengend Snippet: Whole-Brain-Scale Mapping of PSD95 and SAP102 (A) PSD95 (green) and SAP102 (magenta) expression in stitched down-sampled images of five coronal sections (S1–S5). Bregma (β) level is indicated. (B) PSD95 and SAP102 synaptome parameters in ARA anatomical subregions. Parameter units are as follows: density, number of puncta per 100 μm 2 ; size, μm 2 ; intensity, mean gray value per punctum (AU × 10 4 ); colocalization, %. (C) Synaptome maps of delineated regions in five sections. Median punctum density (i), intensity (ii), size (iii), and colocalization (iv) for PSD95 (top) and SAP102 (bottom) are shown. Parameter units: density, number of puncta per 100 μm 2 ; intensity, mean gray value per punctum (AU); size, μm 2 ; colocalization, %. (D) PSD95 punctum intensity in delineated subregions of the hippocampus and cortex. CA1, cornu ammonis; CX, isocortex; DG, dentate gyrus. (E) SAP102 punctum intensity in delineated subregions of hippocampus and cortex. (F) Similarity matrix between pairs of subregions (rows and columns). White lines outline three major blocks (cerebrum, brainstem, and cerebellum). Pink box highlights hippocampal subregions. (G) Dendrogram showing hierarchical organization of subregions based on their similarity. Branch tips represent delineated subregions, colored as in (B).

Article Snippet: Triple Spots Colocalization – Imaris Plugin , Bitplane , http://open.bitplane.com/tabid/235/Default.aspx?id=91.

Techniques: Expressing

Journal: Neuron

Article Title: Architecture of the Mouse Brain Synaptome

doi: 10.1016/j.neuron.2018.07.007

Figure Lengend Snippet:

Article Snippet: Triple Spots Colocalization – Imaris Plugin , Bitplane , http://open.bitplane.com/tabid/235/Default.aspx?id=91.

Techniques: Western Blot, Virus, Recombinant, Plasmid Preparation, Sequencing, Software, Comparison